大豆球蛋白对仔猪小肠上皮细胞Occludin mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2013.08.012

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (8): 1258-1262.doi: 10.11843/j.issn.0366-6964.2013.08.012

大豆球蛋白对仔猪小肠上皮细胞Occludin mRNA表达的影响

韩蕊^1,2，赵元^1,2，潘丽^1,2，王雨琼^1,2，秦贵信^1,2*

(1. 吉林农业大学动物科学技术学院，长春 130118; 2. 吉林农业大学动物生产及产品质量安全教育部重点实验室，长春 130118)

收稿日期:2012-12-04 出版日期:2013-08-23 发布日期:2013-08-23
通讯作者: 秦贵信,教授,E-mail:guixinqin@jlau.edu.cn
作者简介:韩蕊(1987-),女,内蒙古呼伦贝尔人,硕士,主要从事比较动物营养学研究, E-mail: hanrui0409@163.com
基金资助:
国家自然科学基金青年基金项目(31101719);省科技发展计划项目青年科研基金(201201098)

Effects of Glycinin on Occludin mRNA Expression in Piglet Intestinal Epithelial Cells

HAN Rui^1,2, ZHAO Yuan^1,2, PAN Li^1,2, WANG Yu-qiong^1,2, QIN Gui-xin^1,2*

(1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2. Key Laboratory of Animal Production & Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China)

Received:2012-12-04 Online:2013-08-23 Published:2013-08-23

摘要/Abstract

摘要：

旨在研究纯化的大豆球蛋白对离体培养的仔猪空肠上皮细胞（IPEC细胞）通透性以及对细胞间紧密连接蛋白Occludin mRNA表达的影响。将不同浓度的大豆球蛋白(0~5.0 mg·mL^-1)与肠上皮细胞共同培养24 h后，检测跨上皮细胞电阻值并用实时荧光定量PCR方法研究Occludin mRNA表达的变化规律。结果表明，与对照组相比，除大豆球蛋白浓度为0.5 mg·mL^-1时无明显变化，其他各浓度跨上皮细胞电阻值和Occludin mRNA相对表达量均呈显著下降趋势（P<0.001）。由此可知，大豆球蛋白使仔猪小肠上皮细胞通透性增加并使Occludin mRNA表达量下降，进一步明确了大豆球蛋白影响细胞增殖且对肠上皮细胞造成直接损伤，并破坏了肠上皮细胞完整性。

Abstract:

This experiment was conducted to evaluate the effects of purified Glycinin on the permeability and the expression of tight junction protein Occludin in piglet intestinal epithelial cells (IPEC). IPEC were cultured with mediums added with Glycinin by different concentrations (0-5.0 mg·mL^-1) for 24 h. Measuring the mRNA expression of Occludin by real-time quantitative FQ-PCR and the transendothelial electrical resistant (TEER). The results showed that, compared with the control group, Glycinin could decrease the TEER and the relative expression of Occludin (P<0.001) mRNA in piglet, but it had no significant effect at the concentration of 0.5 mg·mL^-1. These results indicate that Glycinin can increase the permeability of the epithelial cells, and decrease the Occludin mRNA expression. Furthermore, this study clarify that the Glycinin directly induces intestinal damage by depressing intestinal epithelial cells growth and damaging the integrity of intestinal cells.

中图分类号:

S828
S811.2

韩蕊，赵元，潘丽，王雨琼，秦贵信. 大豆球蛋白对仔猪小肠上皮细胞Occludin mRNA表达的影响[J]. 畜牧兽医学报, 2013, 44(8): 1258-1262.

HAN Rui, ZHAO Yuan, PAN Li, WANG Yu-qiong, QIN Gui-xin. Effects of Glycinin on Occludin mRNA Expression in Piglet Intestinal Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1258-1262.

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大豆球蛋白对仔猪小肠上皮细胞Occludin mRNA表达的影响

Effects of Glycinin on Occludin mRNA Expression in Piglet Intestinal Epithelial Cells

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